Xuan Feldman
New York University
Investigating the Influence of m6A on lncRNA Structural Dynamics and Stability in vivo
Long, non-coding RNAs (lncRNAs) undergo modifications, enabling them to play a crucial role in the regulation of many biological processes and, as such, have become important in understanding many aspects of cancer and disease pathways. However, our understanding of the interplay between m6A, the most common modification, and lncRNA’s function and structure is poorly understood. This work aims to determine how lncRNA structure is related to m6A modification and if those dynamics correlate to transcript stability. Here, we describe the initial results of the project, which lay the foundation for structural dynamics studies. m6A writer proteins were knocked out to determine the impact that m6A modification has on lncRNA abundance levels. So far, m6A appears to have both a positive and negative influence on lncRNA transcript abundance. These preliminary results lay the foundation for future work that will investigate how base modified structures contribute to stability. Overall, this project contributes to our understanding of the relationship between lncRNA structure, modification, and function in the context of cellular function and disease.
Long, non-coding RNAs (lncRNAs) undergo modifications, enabling them to play a crucial role in the regulation of many biological processes and, as such, have become important in understanding many aspects of cancer and disease pathways. However, our understanding of the interplay between m6A, the most common modification, and lncRNA’s function and structure is poorly understood. This work aims to determine how lncRNA structure is related to m6A modification and if those dynamics correlate to transcript stability. Here, we describe the initial results of the project, which lay the foundation for structural dynamics studies. m6A writer proteins were knocked out to determine the impact that m6A modification has on lncRNA abundance levels. So far, m6A appears to have both a positive and negative influence on lncRNA transcript abundance. These preliminary results lay the foundation for future work that will investigate how base modified structures contribute to stability. Overall, this project contributes to our understanding of the relationship between lncRNA structure, modification, and function in the context of cellular function and disease.